CircularLigate simulates ligation of all possible fragment combinations into circular plasmids.

CutWithEnzyme cuts a given sequence with an enzyme represented by an Enzyme struct.

CutWithEnzymeByName cuts a given sequence with an enzyme represented by the enzyme's name.

GoldenGate simulates a GoldenGate cloning reaction.

Enzyme is a struct that represents restriction enzymes.

Fragment is a struct that represents linear DNA sequences with sticky ends.

Overhang is a struct that represents the ends of a linearized sequence where Enzymes had cut.

Part is a simple struct that can carry a circular or linear DNA sequence.